Composite

Part:BBa_K113005:Design

Designed by: Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)


pLac->tetR->pT7->tetR operator ->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 931
    Illegal BsaI.rc site found at 1654


Design Notes

We combined BBa_K113003 and BBa_K113004 by fusion PCR. Since the efficency is very low, we tried to use standard assembly to ligate them together.


Source

BBa_K113003 BBa_K113004

References